STUDY OF TOXINS OF CLOSTRIDIUM BOTULINUM III
نویسندگان
چکیده
منابع مشابه
Molecular composition of Clostridium botulinum type A progenitor toxins.
The molecular composition of progenitor toxins produced by a Clostridium botulinum type A strain (A-NIH) was analyzed. The strain produced three types of progenitor toxins (19 S, 16 S, and 12 S) as reported previously. Purified 19 S and 16 S toxins demonstrated the same banding profiles on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), indicating that they consist of the ...
متن کاملMolecular construction of Clostridium botulinum type A toxins.
Two Clostridium botulinum type A toxic fractions, named large (L) and medium (M) toxins, were eluted from Sephadex G-200. Sucrose density gradient centrifugation resolved L toxin (2.5 X 10(8) to 3.0 X 10(8) mean lethal doses per mg of N) into two fractions, 19S and 16S. The same procedure performed at pH 8resolved it into three fractions; the heavier two were both nontoxic and hemagglutinin pos...
متن کاملComparison of toxins of Clostridium butyricum and Clostridium botulinum type E.
The toxin of Clostridium butyricum strains isolated from two infants with botulism is neutralized by antitoxin for type E botulinum toxin. This toxin and that of a C. botulinum type E strain were purified by the same protocol. Both toxins were Mr 145,000 proteins which, when activated with trypsin, were composed of an H subunit of Mr 105,000 and an L subunit of Mr 50,000. The activated specific...
متن کاملStudies on immunity to toxins of Clostridium botulinum. III. Preparation, purification, and detoxification of type E toxin.
Although type E strains of Clostridium botulinum have been implicated in human botulism since 1936, no studies of the isolation or characterization of the toxin have been reported. Crude toxoids have been prepared by various workers for immunization of animals and preparation of antitoxin. Barron and Reed (1954) presented a systematic study of methods for preparation of crude alum precipitated ...
متن کاملDiffusion of Botulinum Toxins
BACKGROUND It is generally agreed that diffusion of botulinum toxin occurs, but the extent of the spread and its clinical importance are disputed. Many factors have been suggested to play a role but which have the most clinical relevance is a subject of much discussion. METHODS This review discusses the variables affecting diffusion, including protein composition and molecular size as well as...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1955
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.70.4.363-367.1955